Biochemistry

Glycoproteomics and Protein Expression

Glycoproteomics is a special branch of proteomics that identifies, catalogs, and characterizes carbohydrate containing proteins after the completion of post-translational modification. Protein expression is the process in which proteins synthesis, regulation and modification occurs in living organisms. Analysis of wide range of structurally diverse proteins by genomic technologies and due to increasing complexity in disease targets, innovative approaches for the purification, expression and characterisation of recombinant proteins has increased over years.

Protein expression is the biotechnological procedure of generating a specific protein. It is achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. This contains the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to exact sub cellular or extracellular locations.